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Registros recuperados : 6 | |
1. | | FANTAZZINI, T. B.; ROSA, S. D. V. F. da; COELHO, S. V. B.; SOUSA, P. H. A.; PEREIRA, D. de S.; VILELA, A. L. de O.; RIBEIRO, F. A. S. Applicability of the tetrazolium test to coffee seeds with different types and levels of stresses. Coffee Science, v. 16, e161904, 2022. Biblioteca(s): Embrapa Café. |
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2. | | SILVA, G. L. da; MEDEIROS FILHO, S.; ZANDAVALLI, R. B.; PEREIRA, D. de S.; SOUSA, G. G. de. Biometria e emergência de Amburana cearensis (Allemão) A.C. Smith em função da coloração do fruto. Ciência Florestal, Santa Maria, RS, v. 23, n. 4, p. 635-642, out./dez. 2013. Biblioteca(s): Embrapa Florestas. |
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3. | | OLIVEIRA, L. A.; SOUZA, G. A. de; SILVA, B. T.; ROCHA, A. A. G.; PICOLI, E. A. de T.; PEREIRA, D. de S.; DONZELES, S. M. L.; RIBEIRO, M. de F.; FERREIRA, W. P. M. Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. Coffee Science, v. 15, e151704, p. 77 -100, 2020. Biblioteca(s): Embrapa Café. |
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4. | | ALMEIDA, T. T. de; ROSA, S. D. V. F. da; OLIVEIRA, J. A.; OLIVEIRA, A. dos S.; SILVA, A. A. da; PEREIRA, D. de S. Influence of tannin on sorghum seed germination. African Journal of Food Science and Technology, v. 5, n. 6, p. 136-142, jun. 2014. Biblioteca(s): Embrapa Café. |
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5. | | ESPERON REIS, R. de G.; GUIMARÃES, R. M.; PEREIRA, D. de S.; CASTRO, M. B. de; VIEIRA, A. R.; CARVALHO, M. L. M. de. Qualidade fisiológica de sementes de berinjela osmocondicionadas submetidas à secagem. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 11, p. 1507-1516, nov. 2013. Título em inglês: Physiological quality of osmoprimed eggplant seeds subjected to drying. Biblioteca(s): Embrapa Unidades Centrais. |
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6. | | SOUZA, A. C. de; COSTA, M. C.; FIGUEIREDO, M. A. de; PEREIRA, C. C.; COELHO, S. V. B.; VILELA, A. L. O.; PEREIRA, D. de S.; ROSA, S. D. V. F. da. Steps of cryopreservation of coffee seeds: physiological responses and antioxidant systems. Ciência Rural, v. 54, n. 2, e20220480, 2024. 12 p. Título em português: Etapas da criopreservação de sementes de café: respostas fisiológicas e de sistemas antioxidantes. Biblioteca(s): Embrapa Café. |
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Registros recuperados : 6 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
01/09/2014 |
Data da última atualização: |
09/12/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MÜLLER, B. S. de F.; SAKAMOTO, T.; MENEZES, I. P. P. de; PRADO, G. S.; MARTINS, W. S.; BRONDANI, C.; BARROS, E. G. de; VIANELLO, R. P. |
Afiliação: |
BÁRBARA SALOMÃO DE FARIA MÜLLER, UFV; TETSU SAKAMOTO, UFMG; IVANDILSON PESSOA PINTO DE MENEZES, INSTITUTO FEDERAL GOIANO, Urutaí-GO; GUILHERME SOUZA PRADO, bolsista CNPAF; WELLINGTON SANTOS MARTINS, UFG; CLAUDIO BRONDANI, CNPAF; EVERALDO GONÇALVES DE BARROS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ROSANA PEREIRA VIANELLO, CNPAF. |
Título: |
Analysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plant Molecular Biology, Dordrecht, v. 86, n. 4/5, p. 455-470, Nov. 2014. |
DOI: |
10.1007/s11103-014-0240-7 |
Idioma: |
Inglês |
Conteúdo: |
The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean. MenosThe increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides mo... Mostrar Tudo |
Thesagro: |
Feijão; Genética molecular; Phaseolus vulgaris. |
Thesaurus NAL: |
Beans; Fabaceae; Genomics; Microsatellite repeats; Molecular genetics; Transcription factors; Transposons. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02734naa a2200337 a 4500 001 1993888 005 2014-12-09 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1007/s11103-014-0240-7$2DOI 100 1 $aMÜLLER, B. S. de F. 245 $aAnalysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs.$h[electronic resource] 260 $c2014 520 $aThe increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean. 650 $aBeans 650 $aFabaceae 650 $aGenomics 650 $aMicrosatellite repeats 650 $aMolecular genetics 650 $aTranscription factors 650 $aTransposons 650 $aFeijão 650 $aGenética molecular 650 $aPhaseolus vulgaris 700 1 $aSAKAMOTO, T. 700 1 $aMENEZES, I. P. P. de 700 1 $aPRADO, G. S. 700 1 $aMARTINS, W. S. 700 1 $aBRONDANI, C. 700 1 $aBARROS, E. G. de 700 1 $aVIANELLO, R. P. 773 $tPlant Molecular Biology, Dordrecht$gv. 86, n. 4/5, p. 455-470, Nov. 2014.
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